CAS scientists published research on protein argonaute-1

Protein argonaute-1(EIF2C1) encodes a member of the Argonaute family of proteins that play a role in polymer interference. EIF2C1 is very basic, and contains a PAZ domain and a PIWI domain. EIF2C1 can act with dicer1 and play a job in short-interfering-RNA-mediated factor silencing. It is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.

Model organisms are employed in the study of EIF2C1 operate. A conditional knockout mouse line, known as Eif2c1tm1a Wtsi was generated as a part of the International Knockout Mouse syndicate program, a high-throughput cause project to generate and distribute animal models of illness to interested scientists. Male and feminine animals underwent a consistent composition screen to work out the results of deletion. Twenty two tests were dole out on mutant mice and 2 important abnormalities were observed: homozygous mutants were subviable and females had belittled current aspartate aminotransferase levels.

EIF2C1 ELISA Kit( www.cusabio.com/ELISA_Kit-76128/ ) employs the quantitative sandwich protein bioassay technique. Protein specific for EIF2C1 has been pre-coated onto a microplate. Standards and samples area unit pipetted into the wells and any EIF2C1 present is certain by the immobilized protein. When removing any unbound substances, a biotin-conjugated protein specific for EIF2C1 is extra to the wells. Then laundry, avidin conjugated peroxidase is extra to the wells. Following a wash to get rid of any unbound avidin-enzyme chemical agent, a substrate resolution is extra to the wells and color develops in proportion to the number of EIF2C1 certain in the initial step. the colour development is stopped and the intensity of the colour is measured.