The most complete family of enzymes function chart

Recently, the US Department of Energy-funded scientific user equipment, bio-energy research cooperation with the three major one center, the development and analysis of the family GH55 cellulase enzyme in a high-resolution crystal structure. Then they use a variety of techniques to further complete the "whole GH family of enzymes by far the most complete functional diagram."

GH55 enzyme family members because of their ability to break down cellulose and well known, and therefore is committed to enhancing the mass production of biofuels bioenergy researchers interested in. The method described in this study may allow researchers to study the whole enzyme families simultaneously, thereby accelerating the process of Cellulose degrading enzymes.

US Department of Energy Joint Genome Institute group (DOE JGI) carried out many micro-organisms and metagenome projects are concentrated in insects and animals gut microflora, because they play a role in the decomposition of plant clumps consumed in the host. When the DOE JGI researchers in the "science" published rumen metagenome data, they are to (CAZymes) database increased by nearly 30,000 candidates cellulose degradation genes - encoding carbohydrate active enzymes.

However, each of these genes in a gene functional annotation, the process can be time consuming. For example, cellulose degrading enzyme gene GH55 family, before most of the work carried out on the fungus. For example, the
Elisa Kit For Food Safety & Drug Residues http://www.cusabio.com/catalog-12-1.html is the use of white rot fungi Phanerochaete chrysosporium inferred from the, DOE JGI has conducted a sequenced.

May 8, 2015, a study published in the "Journal of BioLogical Chemistry", the research team (including researchers from DOE JGI and the US Department of Energy-funded Great Lakes Bioenergy Research Center), characterized another GH55 protein - -SacteLam55A structure and function. SACTE_4363 gene encoding this protein is separated from the pine wasp Sirex noctilio gut microbes SirexAA-E out. When the researchers cultured microbes on cellobiose, xylan, and pretreated switchgrass samples, we found that the gene, so that it has a cellulolytic nature.

In order to determine the structure of the gene, the researchers rely on the Advanced Photon Source (Advanced Photon Source) diffraction data collection, development of high-resolution crystal structure. Through a variety of analytical and technical (such as gene synthesis and cell-free protein translation), the research team is also able to characterize the biochemical properties and structure GH55 family.

The research team reported that: "To our knowledge, combined with gene synthesis, cell-free translation and testing, use the entire GH55 substrate diagnostics panel, representing the entire GH family by far the most complete functional spectrum." Two DOE user facilities and DOE Bioenergy Research Center, co-led the study, a combination of different techniques that will allow us to understand the structure and function of cellulose, beyond any depth capability of a device.