The new mechanism programmed to induce iPS heavy GATA proteins

GATA family of proteins plays an important role in the lineage differentiation and transdifferentiation process. Before large-scale screening study found that cell reprogramming factors OCT4 dry crucial factor can be regulated within the development and differentiation of mesoderm (ME) (such as GATA3, GATA6, PAX1) instead, but the lineage-specific clues Contact and GATA proteins and activation of pluripotent how to adjust the balance between the two.

The study found that all of the GATA family members (GATA1-GATA6) can replace Oct4 induced pluripotent cells, and induction of the time was different phases of the cell reprogramming gene expression profiles during the analysis found that GATA proteins can inhibit the ectoderm lineage gene thereby increasing the expression of cell fate toward pluripotency. By studying the domain of GATA family of proteins found in the C-terminal zinc finger structure of a critical conserved DNA-binding domain of the GATA family members induce pluripotency. The use of RNA-Seq and ChIP-Seq comprehensive analysis, the researchers found that pluripotency-associated factor GATA family members Sall4 is induced reprogramming directly target and as a bridge between the lineage differentiation factor GATA members can link up role pathway.

This study illustrates the important role of GATA proteins induce iPS reprogramming and internal mechanisms, but also prompted Mouse Estradiol ELISA Kit http://www.cusabio.com/ELISA_Kit-75683/ in cell fate transformation process as the media plays an important role in laying the foundation for future research, the research results published in Cell Research.