Reactive nerve cells have firstly been cultured!

The official website of University of Pennsylvania School of Medicine has just announcement on17th that research team from the hospital James Abbe for the first time used the surgical excision of the brain tissue in the laboratory to culture adult nerve cells and to identify five types of brain cells and each cell synthesis of proteins. The study using recombinant human proteins has been published in the issue of Cell Reports this week.

The brain tissue in this trial did not contain tumor cells from seven patients, including three patients with epilepsy who received temporal lobe surgery after temporal lobectomy and four patients with glioblastoma after surgery. The researchers from excised tissue was picked out without any tumor cells used in part. When these tissue samples were obtained, they were immediately treated with papain, a proteolytic enzyme, to produce active neurons. They analyzed more than 300 active cells one after the other and identified five known brain cell types: oligodendrocytes, microglia, neurons, endothelial cells and astrocytes.

Because it is not possible to study individual neuronal cells in adult patients, human brain disease awareness and development-related therapies have been slow. "It's amazing how we can nurture nerve cells, and we're finally going to have a mystery about the most mysterious organism in the body," says Abbein. "Previous studies of geriatrics rely primarily on animal models, but this time, Neurocytes originate in different age groups from the age of 20 to 60 years and can be used to detect the difference of molecular markers between the nerve cells of the elderly and the young people so as to provide a new research system of geriatric diseases."

The team used RNA sequencing to find more than 12,000 genes in these cells, and each has hundreds of different RNAs. They also identified long-chain non-coding RNAs corresponding to different cell types, resulting in a completely different gene expression in each patient's nerve cells, which provided strong support for personalized medicine. The researchers said that the new study on the use of cell replacement therapy to repair damaged brain tissue "repair neurosurgery" is of great significance. It is expected to make this therapy as soon as possible in human clinical trials. Flarebio offers high-quality recombinant proteins like recombinant Cdh11 for you research.