BIOABB Assay Designs

BIOABB: A leading provider of immunoassay kits, antibodies, and reagents to the life science and translational research markets, has announced the release of the first commercially available multiplex assay dedicated to the analysis of heat shock proteins and molecular chaperones, the MultiBead(TM)* HSP/Chaperone 8-Plex Kit.

The bead-based multiplex immunoassay enables measurement of HSP client proteins (Akt and Akt phosphor-Ser473) and HSPs (Hsp27 phospho Ser15, Hsp27 phospho-Ser82, Hsp40, Hsp60, Hsp70 and Hsp90 alpha) in cell lysates. The assay utilizes monoclonal antibodies or antigen affinity purified polyclonal antibodies covalently coupled to latex beads. The detection antibodies are conjugated to biotin followed by a streptavidin-PE conjugate and analyzed on a dual-laser flow cytometer. The HSP/Chaperone panel provides a sensitive, rapid and specific method for accurately determining analyte levels in cell lysates.

The MultiBead technology, which already has products for inflammatory markers, is a major focus for Assay Designs research and development department and several additional panels are expected to launch this calendar year.

Additionally, Assay Designs has made available a complete update of the MultiBead Analysis Software in releasing version 2.0. This newest iteration of the MultiBead software has a more intuitive and graphical interface which complements additional features including the usage of dot-density scatter plots, the ability to change and recalculate experimental parameters in real time, and more extensive result reporting with customizable data output. The new software package also allows the user to create experimental templates for streamlining analysis of similar experiments, as well as the ability to save progress at defined points in the analysis so it can be completed or recalculated at another time. MultiBead Analysis Software v2.0 is available for download at no charge to customers from the Assay Designs website.

Based in BIOABB( www.bioabb.com ), Assay Designs develops and manufactures immunoassay (ELISA) kits, luminescent reagents, antibodies, proteins, and extracts used for life sciences research. The company markets these products under the Assay Designs and Stressgen(R) brand names. Researchers use the BIOABB products to detect and quantify molecules that are important in inflammation, cell signaling, and cellular stress. New assay platforms include MultiBead(TM)* bead-based multiplex immunoassay kits and ImmunoSet(TM) ELISA development reagents.