Researchers from the Shanghai Institute of Health Sciences, Shanghai Jiaotong University School of Medicine recently used the human genome-wide transcription factor siRNA library to screen transcription factors which participate in the ESC self-renewal and found that a series of important genes which are important for maintaining human ESC, particularly the great importance and mechanism of PHB as a new member of HIRA complex in the regulation of human embryonic stem cells (embryonic stem cells, ESC) self-renewal. The study was published in the December 8 issue of Cell Stem Cell which also has other studies on recombinant human proteins, and the author of the article is Professor Jin Ying from Shanghai Jiaotong University School of Medicine.

In this study, the researchers used the human genome-wide transcription factor siRNA library to screen participants in the ESC self-renewal transcription factor and found a series of genes that play an important role in maintaining human ESC properties. They studied the PHB gene in particular. Previous studies have learned that PHB is involved in a variety of important life processes in mammalian cells. However, the function of the gene in the ESC has not been reported. The study of the Jinying group found that PHB plays an important role in maintaining human ESC self-renewal and promoting the reprogramming of human adult cells, especially PHB plays a unique role in maintaining the correct histone methylation of human ESC effect. Further studies have shown that PHBs interact with the HIRA complex of the chaperone protein H3.3 and maintain the protein stability of the HIRA complex.

In addition, the researchers found that PHB and HIRA jointly regulate the enrichment of H3.3 in the whole genome of chromatin, especially in the regulation of H3.3 in isocitrate dehydrogenases (IDHs) Gene promoter region and the expression of IDH gene to control the production of a-ketoglutarate (a-KG), a key metabolite that plays an important role in the fate of ESC, to shape the correct histones Methylation levels, maintenance of human hESC self-renewal and epigenetic characteristics.

This study reveals the key role of PHB in pluripotency regulation through genome-wide, large-scale siRNA screening, and it reported that PHB interacts with HIRA complex to regulate the enrichment of H3.3 on chromatin. This study also revealed the important regulation of HIRA complex and H3.3 on IDH gene expression and key metabolite a-KG, and proposed an apparent-metabolic regulatory loop maintained by human ESC. Flarebio provides you with good-quality recombinant proteins like recombinant ECEL1 at competitive prices.