Japanese scholars achieve the ultimate dream of Systems Biology

Japan's RIKEN and Tokyo University scientists will be organized decolorization and combine light cut fluorescence microscope imaging a variety of organs and the whole organism, has been extremely detailed internal images. The results published in this issue of the journal Cell.

Understanding of life's modus operandi is always the ultimate dream of systems biology. The tissues and organisms transparent and accurate imaging of single-cell resolution, will become a new way to achieve this dream. Maybe you will choose a good Mouse Cystatin C ELISA Kit from:http://www.cusabio.com/ELISA_Kit-73147/

In this study, the researchers used a method called CUBIC, and they have used this method before imaging the entire brain. Lipid-rich brain tissue more easily transparent, but in many other parts of the body can absorb light containing chromophore (chromophore), such as heme-heme. Heme is a vital component of hemoglobin present in the vast majority of the body tissues, and will block the light.

CUBIC need to use reagent fixed tissue, it is not used for living organisms. However, CUBIC can help people understand the 3D structure of the organ, as well as the expression of specific genes in different tissues, the first author Kazuki Tainaka said. "Our approach can be almost transparent body pups and adult mice. With this technology people can observe the organization of the cellular network, which is a fundamental challenge to the field of biology and medicine."

"CUBIC can be used for 3D pathological studies, anatomy studies and immunohistochemical analysis of the entire organism. For example, you can use CUBIC observe the development of the embryo at the cellular level, or the development of cancer and autoimmune diseases. In-depth understanding course of the disease will help us to develop new therapeutic strategies. at the single cell level whole-body imaging, is expected to achieve the ultimate dream of the field of systems biology, "the study's leader Hiroki Ueda said.

The research team is being further improved microscopic imaging method for fast imaging of the human brain and even the whole body of adult mice. They hope that through this technology, in-depth understanding of the mechanisms of autoimmune diseases and mental illness.