The visual observation of X chromosome inactivation

In this study, the researchers bred inducible Cre recombinase expression of a fluorescent reporter protein --- green fluorescent protein (green fluorescent protein, GFP) in the nucleus, or what is known as a red fluorescent protein tdTomato --- mice transgenic varieties. Using a mouse species each species a tissue-specific promoter to drive the expression of Cre, and the expression of GFP expressing mouse strains of mice after propagation tdTomato breed heterozygous female mice, and heterozygous females in each two X chromosomes of mice, one X chromosome from the GFP-expressing mouse strains, one from another species of mice expressing tdTomato. They analyzed the green fluorescence and red fluorescence in each cell expression of Cre found in X chromosome inactivation methods are very different between different organizations, sometimes Mouse Platelet Factor 4 ELISA Kit http://www.cusabio.com/ELISA_Kit-95923/ exhibit unique about asymmetry. Different ways of X chromosome inactivation leads to retinal vascular disease X-linked differences in the symptoms, but also cause central nervous system of biological diversity.

Nathans said, "The brain is the diversity of the really important things." He said, different gene expression patterns can help the brain process information. In this study, the researchers propose this method can be used in accordance with a variety of different ways, including the development of visualization when the mouse X chromosome inactivation observed, sorted and carry the mutation because active X chromosome in the cell and X chromosome carrying the normal cells, and compare the differences between these two types of cells in gene expression.

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