2014年12月8日星期一

Open source qPCR: Everyone can do DNA diagnostics

English full name is Real-time Quantitative PCR Detecting System, namely real-time quantitative nucleic acid amplification detection system, also known as gene amplification in real-time quantitative fluorescence detection system, which refers to the polymerase chain reaction PCR.

Real-time PCR thermal cycler (Real-Time PCR) is a powerful technique that can detect this type of E. coli and listeria foodborne contaminants can diagnose AIDS (HIV) and malaria such infections, currently raging Ebola virus on the African continent can also use it to help diagnose. Selective breeding of plants and animals, monitoring water quality and found that the genomic DNA mutations that may occur, these problems can be easily resolved qPCR. qPCR can help humans earlier completion of the Human Genome Project, in the near future, perhaps immortality is no longer a luxury.

However, the conventional PCR machine costs up to $ 2,000, in those places really need, it is hard to afford its high cost. In order to change this situation, Chai Biotechnologies from California begun making more Mouse Estradiol ELISA Kit http://www.cusabio.com/ELISA_Kit-75683/ . In 2010, the founder Josh Perfetto and Tito Jankowski start on Kickstarter to raise the public, and successfully delivered the world's first open-source real-time PCR thermal cycler. Today, about 800 people around the world in the PCR was required to use the. Four years later, Chai Biotechnologies again embarked on Kickstarter, this time, Josh set up an include electrical, mechanical, optical and software engineers, including a strong R & D team, and their goal is to qPCR upgrade to a more professional level, not only only copy DNA, but also to convert it into data.

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