The University of Tokyo in Japan announced on October 11th in 2016 that they have successfully developed a new technology which can efficiently control the DNA recombination reaction by conducting short-term exposure to weak light and using recombinant horse proteins.
Associate Professor Okazaki Yasuke, Associate Professor Takashi Sato from Graduate School of Comprehensive Cultural Studies, the University of Tokyo and Professor Yazawa Shinya of the Department of Rehabilitation and Rehabilitation Medicine of Columbia University, Yasuyuki Yasuo, and other researchers have completed the related research. Specific results have been published in the October 10th issue of the well-known US scientific journal Nature Chemical Biology.
Cre-LoxP recombinase system technology is used by most researchers to knock out the base sequence of a target gene from the genome or to insert a gene into the genome. In recent years, the use of compounds and light to artificially control the Cre-LoxP recombinase system has attracted more and more attention. In particular, if the Cre-LoxP recombinase system can be controlled by light, then targeting specific tissues and cells at any time point to induce DNA recombination will be realized. However, the existing technology uses light control but the efficiency of DNA recombination is significantly reduced, which is a major problem consistent with the researchers.
In this study, the panelists connected light-activated protein recombinase (Cre) which was temporarily inactive and successfully developed a light-activated Cre recombinase that controls the recombination of DNA by light-ray irradiation. The enzyme was named PA-Cre. PA-Cre is capable of producing light recombination with very low light (one hundred thousandth of the light intensity commonly used in light genetics) and a short time of about 30 seconds is enough to induce DNA recombination. In addition, the researchers have successfully used PA-Cre to conduct DNA recombination on the selected target site.
On this basis, the researchers applied PA-Cre to the genetic gene in the deep of mouse body to conduct light control. It has been found that the use of a non-invasive method for light irradiation from the outside of an organism can induce DNA recombination efficiently in the liver of a mouse's deep part of the body. The researchers said that the results enable the genetic activities within the living body can be controlled by non-invasive light irradiation, which is of great significance in proving the function of the disease-related gene. Flarebio offers good-quality recombinant proteins like recombinant CDH11 at competitive prices.
没有评论:
发表评论