The tumors’ condition can be distinguished partly from miRNA Expression in Mucinous Appendiceal Neoplasm

Mucinous adenocarcinoma of the appendix is a rare case. Differentiation mucinous carcinoma from mucinous cystadenoma is very challenging, depending on the establishment of the appendix wall in the presence of malignant cells. In some cases, the invasion may be difficult, especially in the early stages of the disease, the patient's condition could have a devastating impact. Therefore, it is necessary to develop a secondary test. Scientists can use it to distinguish mucinous cystadenoma and mucinous adenocarcinoma. So far, there is no relevant difference in miRNA expression in the diagnosis of appendiceal mucinous adenocarcinoma of the report.

Recently, a study led by researchers at Wayne State University School of Medicine has been completed. In the research, six confirmed mucinous adenocarcinoma of the appendix and twelve selected cases of mucinous cystadenoma of the appendix. All RNA extracted from the above-mentioned cases of formalin-fixed paraffin-embedded specimens. Comprehensive miRNA microarray gene expression RNA samples from these two institutions merged to analyze, detect differential expression of miRNA of mucinous adenocarcinoma. MiRNA best seven differentially expressed in individual cases, by quantitative reverse transcription polymerase chain reaction (quantitative RT-PCR) for verification.

The LC Sciences miRNA microarray analysis revealed mucinous adenocarcinoma is 646 differentially expressed miRNA. These differences in miRNA expression, the expression of 80 miRNA difference was statistically significant (P <0.01). Quantitative RT-PCR to verify the expression of miR-1, miR-4328 is significantly lower compared to mucinous adenocarcinoma, mucinous cystadenoma (P <0.05). In another aspect, miR-200b in, miR-200c in, miR-451, miR-223 and miR-21, their expression in mucinous adenocarcinoma (P <0.05) were significantly increased.

The researchers observed miRNA detection appendix mucinous adenocarcinoma samples significant change compared to the expression level of mucinous cystadenoma. These data suggest that in mucinous tumors of the appendix of miRNA expression in cancer helps distinguish benign form of supplementary assessment.

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