Analysis of regulation of anthocyanin metabolism in

In order to analyze the regulatory network of anthocyanin metabolism in reddishviolet-shoot tea, Isobaric Tag for Relative and Absolute Quantification (iTRAQ) was carried out for profiling protein expression changes from the purple and green leaves of 'Zijuan' tea (Camellia sinensis var. kitamura). Using this technique, 815 differentially expressed proteins were identified, among which 20 proteins were associated with regulation of anthocyanin metabolism. Compared with green leaves, in purple leaves of 'Zijuan' tea, under the regulation of transcription factor bHLH nd HY5, the transcription activities of genes related to anthocyanin synthesis including chalcone synthase (CHS), chalcone isomerase (CHI), dihydroflavonol 4-reductase (DFR) and anthocyanins synthetase (ANS) were increased, improving amount of proteins, which was conducive to the synthesis of anthocyanin; Anthocyanin was accumulated through the regulations of anthocyanidin 3-O-glucosyltransferase (UFGT) and ABC transporter in cells; The up-regulations of bifunctional 3-dehydroquinate dehydratase (DHQ) and chorismate mutase (CM) revealed that they suppliede enough substrates for anthocyanin synthesis; The activity of lignin synthesis was reduced under the down-regulations of Cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (CAD), peroxidase (POD) and Laccase (LAC), resulting in more intermediate metabolites flowing into anthocyanin synthesis pathway. The study revealed the regulatory network of anthocyanin metabolism in 'Zijuan' tea. Read more:http://www.cusabio.com/Polyclonal-Antibody/UBE2C-Antibody-11098184.html