Electrochemical Aptasensor for the Detection of Ochratoxin A Based on DNA Super-Tree Structures

A paper published recently shows that a nonlinear hybridization chain reaction, magnetic separationand printed electrodes were used to construct an electrochemical aptasensor for the sensitive detection of OTA based on DNA super-tree structures. First, capture probes were accurately modified on the surface of MBs and hybridized with the added partly complementary OTA aptamer probe, constituting a magnetic sensor platform of analysis, separation and detection. In the presence of OTA, a series of non-linear hybrid chain reaction could occur upon adding of substrate probes and auxiliary probes, resulting in the formation of DNA super-tree structures. Finally, the MBs were adsorbed onto the surface of printed electrode for electrochemical detection. Under optimal conditions, the detection limit was up to 8 fg / mL. This method was expected to provide an efficient, fast, easy, economical and portable way for mycotoxins detection, and would guarantee the quality and safety of agricultural products. YOU MAY LIKE THIS>>>http://www.cusabio.com/Clone/CT0239-1089564.html