The accuracy of CRISPR/Cas9 is improved by new technology

It's great news that scientists from the University of Massachusetts Medical School have developed a new CRISPR/Cas9 technology which is precise enough to surgically edit DNA at nearly any genomic location. The study was published in Nature Methods. The new technology can avoid potentially harmful off-target changes typically seen in the current CRISPR gene editing techniques. Researchers now have created an additional proofreading step by pairing the CRISPR/Cas9 system with a programmable DNA-binding domain (CRISPR/Cas9-pDBD). The new step can improve the accuracy of the gene editing system and open up the way to potential clinical and gene therapy applications. The standard CRISPR/Cas9 systems are breakthroughs in the genome, which is suboptimal for most gene therapy applications involving the editing of massive cells where minimizing collateral damage to the genome is critical. Then the scientists added the extra proofreading step to the system. It now verifies an additional genetic feature in its intended target site before it will cut the genome. It will improve the precision by nearly 100 fold. The researchers have been developing this nuclease platform to excise latent HIV provirus from the genome of infected cells and potentially correct the genetic mutation that leads to chronic granulomatous disease. The CRISPR/Cas9 system can be reprogramed with artificial guide RNAs to cleave sequences within mammalian genomes and insert new fragments of genetic information into cells. CRISPR/Cas9 is such a simple and efficient way of editing the genome to revolutionize biomedical research by being easier to inactivate or activate genes in a cell line for study. The same work which used to take months or years now can be completed in a short time. You may also like these elisa kit products:http://www.cusabio.com/ELISA-Kit/Goat-Apolipoprotein-B-APOB-ELISA-kit-1042036.html