Inhibit the development of lung cancer by silencing a gene

Scientists believe that the discovery of the like from bacteria to plants, insects and human organism "conservative" genes among species play an important biological function. Previous limited research on these genes in a gene --Nitrilase 1 (NIT1) showed that it can inhibit the development of cancer. However, the researchers Thomas Jefferson University Sidney Kimmel Cancer Center found that compared with normal cells, Nit1 in common lung cancer cells is a significant overproduction. When Nit1 silenced, lung tumor growth was inhibited.

Their findings are published in the journal Oncotarget, the first description of the growth and progress Nit1 non-small cell lung cancer role. Senior researcher of the study, radiation oncologist Thomas Jefferson University School of Medicine, Sidney Kimmel Lupo (transliteration, Bo Lu), said these findings strongly suggest that, Nit1 may be a new target for drug treatment required.

Dr Lo said, "Now, in the majority of patients with lung cancer, cancer of the existing therapy is becoming increasingly resistant, making lung cancer as the most important cause of cancer death in the world. Therefore, urgent need for new drugs, Nit1 inhibitors may represent a new drug policy. "Dr. Sidney Kimmel Thomas Jefferson University school of medicine, Radiation Oncology, Pharmacology and experimental Therapeutics Professor Adam Dicker noted that the study is a good example of "aimed at understanding the basic mechanisms of lung cancer Research on how to determine possible new drug targets".

Dr. Lu and his colleagues have prepared a mouse model, when the mouse genome in the presence or absence of Nit1, due KRAS mutations and to develop lung cancer. Using a mouse model lacking Nit by researchers Dr. Jianke Zhang prepared scientists will cross these mice and found that due to the lack of Nit1 tumors, tumors than the mice carrying the gene active Nit1 formed five times smaller. They also found that, Nit1 in human lung cancer tissues and cell lines are highly expressed in cancer cells and silence Nit1, you can reduce the survival of cancer cells.

The researchers then tested the "suppression Nit1 whether to increase cisplatin (a commonly used cancer chemotherapy drugs) in mice with lung cancer in effect". Dr Lo said, "When Nit1 is silenced, the cancer becomes significantly more sensitive to cisplatin. This is the story of discovery - a false assumption brought a possible new drug strategy."

Currently, Dr. Lu and his colleagues are continuing to study Nit1 expression and inhibitory mechanisms behind its potential impact on the development of lung cancer in immune surveillance.

http://www.cusabio.com/ELISA-Kit/Fish-Apolipoprotein-EAPOE-ELISA-kit-1035336.html