Scientists firstly use fluorescent probe to analyse the mystery of cytomembrane

Recently, scientists from Japan, the United States and India firstly observed the raft domain in cytomembrane in live cells, namely the active site carrying special molecular community in cytomembrane. Related study is published in the international journal Nature Chemical Biology. A researcher Naoko Komura said in the study that the speculation of the raft domain with special cell membrane structure domain has gone for more than 25 years, but scientists had never really observed into the structure in living cells, while the researcher made major breakthrough in this study.

In the study, researchers observed the behavior of ganglion monoglyceride. Ganglion monoglyceride is a special kind of lipid molecules, which plays an important role in a variety of biological processes in the body, including the formation of lipid raft domains, the entrance of toxins into cells and receiving chemical signals. However, scientists are still not exactly sure about the working mechanism of ganglioside, so researchers lack for probe to accurately track the movement of ganglioside till now.

In the previous study, researchers were not able to simulate the behavior of a natural ganglioside in artificial model systems, while researchers adsorbed a fluorescent label on a ganglioside in this research, then they took advantage of adsorption fluorescent markers which were absorbed in specific sites synthesized to compound four holistic ganglioside; using this new type of fluorescent analogs and with the combination of unique high-resolution fluorescence molecular imaging technology, researchers have achieved the record of special ganglioside in living cells for the first time.

Researchers reveal how to form ganglioside CD59 protein carry cholesterol and lipid raft region, CD59 GPI-anchored receptor proteins belong to the family of one; Dr. Kenichi Suzuki said the technology is difficult to observe the use of normal fat cells the dynamic behavior of mass raft region, and in this paper we use fluorescent probes ganglioside achieve this purpose, many scientists now can begin to conduct in-depth studies to reveal how toxins, bacteria and viruses invade to cells.

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